Device

Part:BBa_K1491032:Design

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-19)

T7 promoter-RBS-RFP-T7 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 763
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFP transcribed and translated with T7 RNA polymerase.


Source

T7 promoter and RBS are BBa_S04423, RFP is BBa_E1010, T7 terminator is BBa_B0016.

References